Enhancement of quality retention of Grifola frondosa fruiting bodies by erythorbic acid treatment

In this paper, the effects of erythorbic acid (EA) treatment with different concentrations on the quality of Grifola frondosa fruiting bodies stored at 4 °C for 27 days were studied by determining the changes in moisture content, weight loss, browning, electrolyte leakage, malondialdehyde (MDA), and nutritional compounds. The activities of polyphenoloxidase (PPO), cellulase and other antioxidant enzymes including superoxide dismutase (SOD), catalase, and peroxidase (POD) were also measured. Results showed that 0.1% EA-treated G. frondosa fruiting body maintained lower weight loss (< 6.0%, w/w), electrolyte leakage (< 45.8%), MDA (< 4.17 µmol kg−1), and higher moisture content (> 90.7%, w/w). Lower activities of PPO (< 72.64 × 103 U kg−1) and cellulase (< 189.86 × 103 U kg−1) in 0.1% EA-treated samples were observed compared with the other treatments. As a stereoisomer of ascorbic acid (AA), EA also could enhance SOD and POD activities of G. frondosa fruiting bodies. Our findings were the first time to evaluate the effect of EA on maintaining quality in G. frondosa fruiting bodies, and proved that low concentrations of EA (especially 0.1% EA, w/v) treatments were beneficial to preserve G. frondosa fruiting body with even higher efficiency than AA treatment. This study paved a foundation for the enhancement of quality retention of G. frondosa fruiting bodies.


  1. Aguirre L, Jesus MF, Catherine BR, Helen G (2008) Modeling browning and brown spotting of mushrooms (Agaricus bisporus) stored in controlled environmental conditions using image analysis. J Food Eng 91:280–286. Scholar
  2. Antmann G, Ares G, Lema P, Lareo C (2008) Influence of modified atmosphere packaging on sensory quality of shiitake mushrooms. Postharvest Biol Technol 49:164–170. Scholar
  3. Autio WR, Bramlage WJ (1986) Chilling sensitivity of tomato fruit in relation to ripening and senescence. J Am Soc Hortic Sci 111:201–204Google Scholar
  4. Cacace JE, Delaquis PJ, Mazza G (2002) Effect of chemical inhibitors and storage temperature on the quality of fresh-cut potatoes. J Food Qual 25:181–195. Scholar
  5. Fan YN, Wu XY, Zhang M (2011) Physical characteristics and antioxidant effect of polysaccharides extracted by boiling water and enzymolysis from Grifola frondosa. Int J Biol Macromol 48:798–803. Scholar
  6. Hyejin P, Gunhee K (2014) Effect of application of rice bran extract on quality of Agaricus Bisporus during storage. Korean J Hortic Sci Technol 32:834–844. Scholar
  7. Jahangir MM, Jiang TJ, Jiang ZH, Amjad M, Ying TJ (2011) Methyl jasmonate enhances postharvest physicochemical and microbial quality of button mushroom (Agaricus bisporus). J Food Agric Environ 9:91–95Google Scholar
  8. Jiang TJ, Luo SS, Chen QP (2010a) Effect of integrated application of gamma irradiation and modified atmosphere packaging on physicochemical and microbiological properties of shiitake mushroom (Lentinus edodes). Food Chem 122:761–767. Scholar
  9. Jiang T, Wang Q, Xu S, Jahangir MM, Ying T (2010b) Structure and composition changes in the cell wall in relation to texture of shitake mushrooms (Lentinula edodes) stored in modified atmosphere packaging. J Soc Chem Ind 90:742–749. Scholar
  10. Jiang T, Feng L, Zheng X, Li JR (2013) Physicochemical responses and microbial characteristics of shiitake mushroom (Lentinus edodes) to gum arabic coating enriched with natamycin during storage. Food Chem 138:1992–1997. Scholar
  11. Khan ZU, Aisikaer G, Khan RU (2014) Effects of composite chemical pretreatment on maintaining quality in button mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biol Technol 95:36–41. Scholar
  12. Kruger NJ (1994) The Bradford method for protein quantitation. In: Walker JM (eds) Basic protein and peptide protocols. Methods in molecular biology, vol 32. Humana Press, New York. Scholar
  13. Lagnika C, Zhang M, Mothibe KJ (2013) Effects of ultrasound and high pressure argon on physico-chemical properties of white mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biol Technol 82:87–94. Scholar
  14. Lanigan RS (1999) Final report on the safety assessment of ascorbyl palmitate, ascorbyl dipalmitate, ascorbyl stearate, erythorbic acid, and sodium erythorbate. Int J Toxicol 18:1–26Google Scholar
  15. Li X, Jiang Y, Li W, Tang Y, Yun J (2014) Effects of ascorbic acid and high oxygen modified atmosphere packaging during storage of fresh-cut eggplants. Food Sci Technol 20:99–108. Scholar
  16. Liu ZL, Wang XY, Zhu JY, Wang J (2010) Effect of high oxygen modified atmosphere on post-harvest physiology and sensorial qualities of mushroom. Int J Food Sci Technol 45:1097–1103. Scholar
  17. Liu ZQ, Zhang XH, Xue YP, Xu M, Zheng YG (2014) Improvement of alcaligenes faecalis nitrilase by gene site saturation mutagenesis and its application in stereospecific biosynthesis of (R)-(−)-mandelic acid. J Agric Food Chem 62:4685–4694. Scholar
  18. Liu ZQ, Dong SC, Yin HH, Xue YP, Tang XL, Zhang XJ, He JY, Zheng YG (2017a) Enzymatic synthesis of an ezetimibe intermediate using carbonyl reductase coupled with glucose dehydrogenase in an aqueous-organic solvent system. Bioresour Technol 229:26–32. Scholar
  19. Liu ZQ, Wu L, Zhang XJ, Xue YP, Zheng YG (2017b) Directed evolution of carbonyl reductase from Rhodosporidium toruloides and its application in stereoselective synthesis of tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. J Agric Food Chem 65:3721–3729. Scholar
  20. Liu ZQ, Wu L, Zheng L, Wang WZ, Zhang XJ, Jin LQ, Zheng YG (2018) Biosynthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate by carbonyl reductase from Rhodosporidium toruloides in mono and biphasic media. Bioresour Technol 249:161–167. Scholar
  21. Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  22. Nielsen SS (2010) Determination of moisture content. In: Nielsen SS (ed) Food analysis laboratory manual (Food Science Texts Series). Springer, Boston. Scholar
  23. Pavel K (2013) A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. J Sci Food Agric 93:209–218. Scholar
  24. Ping LF, Chen XY, Yuan XL, Zhang M, Chai YJ, Shan SD (2017a) Application and comparison in biosynthesis and biodegradation by Fusarium solani and Aspergillus fumigatus cutinases. Int J Biol Macromol 104:1238–1245. Scholar
  25. Ping L, Wang M, Yuan X, Cui F, Huang D, Sun W, Zou B, Huo S, Wang H (2017b) Production and characterization of a novel acidophilic and thermostable xylanase from Thermoascus aurantiacu. Int J Biol Macromol 109:1270–1279. Scholar
  26. Ping LF, Guo Q, Chen XY, Yuan XL, Zhang CR, Zhao H (2017c) Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain. 3 Biotech 7:56. Scholar
  27. Ping LF, Zhang CR, Cui H, Yuan XL, Cui JT, Shan SD (2017d) Characterization and application of a newly isolated pyrene-degrading bacterium, Pseudomonas monteilii. 3 Biotech 7:309. Scholar
  28. Soliva RC, Elez P, Sebastia MM, Martin O (2000) Evaluation of browning effect on avocado purée preserved by combined methods. Innov Food Sci Emerg 1:261–268. Scholar
  29. Sreenivas KM, Singhal RS, Lele SS (2012) Chemical pretreatments and partial dehydration of ash gourd (Benincasa hispida) pieces for preservation of its quality attributes. LWT Food Sci Technol 44:2281–2284. Scholar
  30. Xing ZT, Cheng JH, Tan Q, Pan YJ (2006) Effect of nutritional parameters on laccase production by the culinary and medicinal mushroom, Grifola frondosa. World J Microbiol Biotechnol 22:1215–1221. Scholar
  31. Xing ZT, Wang YS, Feng ZY, Tan Q (2008) Effect of different packaging films on postharvest quality and selected enzyme activities of Hypsizygus marmoreus mushrooms. J Agric Food Chem 56:11838–11844.
    Google Scholar  CrossRef

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